Degradation of C-terminal tag sequences on domain antibodies purified fromE. colisupernatant
نویسندگان
چکیده
منابع مشابه
Degradation of C-terminal tag sequences on domain antibodies purified from E. coli supernatant
Expression of recombinant proteins often takes advantage of peptide tags expressed in fusion to allow easy detection and purification of the expressed proteins. However, as the fusion peptides most often are flexible appendages at the N- or C-terminal, proteolytic cleavage may result in removal of the tag sequence. Here, we evaluated the functionality and stability of 14 different combinations ...
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ژورنال
عنوان ژورنال: mAbs
سال: 2014
ISSN: 1942-0862,1942-0870
DOI: 10.4161/mabs.36211